Even a severely damaged kidney may be capable of producing sufficient urine volume to prevent salt and water overload and avoid the need for UF.
We calculated that only a very small seepage of ∼2G L/yr (∼0.03% of the FM water volume) is sufficient to maintain current salinity conditions.
cDNA in 10 μL was combined with 2 μL 10× One-Phor-All Buffer (Amersham Biosciences, Piscataway, NJ), 0.6 U DNase I/μg cDNA (Amersham Biosciences), plus sufficient water for 20 μL total volume, and incubated 10 minutes at 37°C.
PCRs for MCA contained PCR buffer, 200 μ M dNTPs, MgCl2 as required (Supplementary Table 1 online), 1 μl LCGreen+ (all Idaho Technologies), 0.25 U of ThermoStart Taq polymerase (ABgene, Epsom, UK), primers as required, DMSO to a final concentration of 5%, 20 ng of genomic DNA and sufficient water to make a final volume of 10 μl.
One of the main messages of Asian Water Development Outlook 2007, a report by the Asian Development Bank, is that proper management, not volume, is the key to maintaining sufficient water supplies in Asia in the face of increasing urbanization, industrialization, population growth, and climate change.
Add sufficient water to cover.
But at least there is sufficient water.
For example, "lack of sufficient water" would then become an aim to "improve water supply".
Sufficient water was supplied throughout.
Is it land area or water volume contaminated?
