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The extruded protoplasm without a cell membrane maintains sufficient viability to regenerate into a mature individual [29].
For the proliferation assay, we used the minimal concentration of FCS (2%) to allow sufficient viability of MDA-MB-231 cells.
Various polymers, such as agar, agarose, alginate, collagen, latex, polyacrylamide, polyethylene glycol diacrylate, and carrageenan, as well as freeze/vacuum drying, have been reported to immobilize cells while retaining sufficient viability [ 25, 33, 43, 49, 51– 51].
For the establishment of this method and its further application it was essential to establish highly reproducible transient transfections in a cell line which showed sufficient viability in the Bionas system for long-term kinetic experiments.
The attenuating radiation dose is known to play an important part in the success of the immunization, in particular over-irradiation leads to a loss in the acquisition of protection, most probably by reducing the number of sporozoites that retain sufficient viability to reach and invade hepatocytes.
Similar(54)
Although the N-terminal residues 1-1955 seem to be deficient in gene silencing, they are sufficient for viability.
Whereas a truncation mutant of the C-terminal 24 amino acids of yeast Hsp90 is sufficient for viability, a truncation of 57 amino acids is not [20].
While this level of suppression does not preclude the residual levels of RabX1 and RabX2 being sufficient for viability, the low levels of most Rab proteins normally makes them rather sensitive to knockdown, and hence these results potentially indicate that RabX1 and RabX2 are probably non-redundant, consistent with their differential location.
Using this plasmid-based Δ FG strategy, we find that a GLFG domain-only pore is sufficient for viability.
While the presence of any of three tyrosines is sufficient for viability and vulval differentiation, fertility is conferred by one specific tyrosine that does not rescue the other Let23 functions [ 13].
Flies carrying only the short arm of the Y chromosome are also viable, but the long arm of the Y is not sufficient for viability in the absence of an X chromosome (Hediger et al. 1998).
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