Sentence examples for sufficient template from inspiring English sources

To ensure sufficient template for repeat testing, virus sequences were first amplified from 5 µL HIV-1 RNA by reverse transcriptase-polymerase chain reaction (RT-PCR) using the reverse primer RTP-REV2 [5'-CTT CTG TAT GTC ATT GAC AGT CC], and forward primer RTP-F1 [5'-CCT CAG ATC ACT CTT TGG CAA CG], which span from n.t. 1 in protease to n.t.

The DNA was extracted using a fast and simple alkaline extraction protocol that provided sufficient template for an early diagnosis.

The 164 reads that appear to have sufficient template DNA are grouped by source clone for further analysis.

This step appends required terminal sequences to each molecule and generates a sufficient template for SOLiD sequencing.

This procedure yields at least 150 μg DNA, i.e. sufficient template DNA for 1000 standard 50 μl PCR reactions (see below).

The amplification protocols utilized here allow buccal cell samples to be used in repeated measures experiments removing the necessity to sample more than once to obtain sufficient template for a single microarray.

Use about 25 ng of plasmid vector per 10 μl reaction, in order to have sufficient templates for the subsequent PCR amplification.

Firstly, the in vivo mitochondrial protein synthesis in the septic ICU patients was not different from that in the control subjects, indicating that sufficient mRNA template existed for translation.

In our opinion, this strategy could be applied when sufficient DNA template is available.

A small reduction in DNA concentration was accepted for the final methodology to allow sufficient PCR template to be available.

While phi29 provides a simplified isothermal method for amplifying limiting material, non-specific DNA fragments of high MW are generated in the absence of sufficient input template.