These results suggested that although Phi suppressed the PSR in Pi starved plants, application of Phi to Pi sufficient plants resulted in activation of PSR.
Phi at lower concentration (≤ 2.5 mM) inhibited root hair formation induced under phosphate starvation; however, 20 mM Phi induced root hair formation in Pi sufficient plants.
The sqRT-PCR experiments confirmed the iron deficient plants had lower expression levels of the transcripts than the iron sufficient plants, replicating the results seen in the microarray data.
Indeed, by the time the panicles appeared (∼40 days after initiation of treatment), the recovering plants had been re-supplied with Pi for ∼20 days, and should thus have a physiological status similar to that of Pi sufficient plants.
Although larger population sizes were used for each cross - to ensure that a well-chosen cross has sufficient plants in its progeny to succeed - the overall size of the breeding programme can be reduced.
Artemis, F2 seeds; Mediplant), three plants were randomly selected as mother plants for clonal propagation by cuttings and sufficient plants were obtained during two rounds of multiplication carried out in a greenhouse.
