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Following treatment, one subgroup of cells was shifted on folate sufficient medium and grown for three more generations.
M. laminosus acquired the most biomass of all the cyanobacteria, at a temperature of 30°C. Figure 2 Growth of Ce and Roman Bath isolates in nitrogen sufficient medium after 12 days, measured as dry weight (g l -1 ).
Thereto, 5-day-old wild type seedlings grown on low but Cu sufficient medium (½ MS supplemented with CuSO4 0.5 μM [ 31]) were incubated for 3 h on liquid ½ MS with tunicamycin [ 32], an inhibitor of N-linked protein glycosylation, or dithiothreitol (DTT), disrupting disulphide bond formation.
The culture was grown for approximately 72 h at 28°C with shaking at 200 rpm and then 200 or 500 ml were transferred to a bioreactor (Braun Biostat CT2-DCU3 or CT5-2, Braunaun Biotech International GmbH, Meisungen, Germany) along with sufficient medium to give total final volumes of 2 or 5 l respectively.
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A Four Seasons spokesman, commenting on its December interest payment, said the company has "sufficient medium-term financial flexibility", adding: "Our residents can be reassured that Four Seasons will continue to make the operational improvements that saw customer satisfaction ratings reach 96 per cent last month".
Drawing here is conceived both as a self-sufficient medium and as a tool useful for the conceptual and practical development of ideas in other media.
The transformants were grown in sulfur-sufficient medium because endogenous ars genes are induced by sulfur deficiency.
At higher inhibitor concentration, however, it is speculated that the growth of M. tuberculosis in iron-sufficient medium is inhibited by sAMS through some other unknown mechanism [ 53].
Although this activity was weak when plants were grown in a standard N-sufficient medium, it was rapidly induced by N-starvation.
Accordingly, we predicted that sAMS had no effect on M. tuberculosis growth in an iron-sufficient medium (see Additional file 1: Section S7). Figure 6B shows that our predictions matched the experimental data under relatively low sAMS concentration (<10 μM).
One system regulates LaPT1 and LaPEPC3 gene expression and acts when P supply is low, and the other system regulates cluster root formation and LaSAP expression and acts even in P-sufficient medium if roots receive a sugar signal.
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