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Except for the needed sufficient incorporation of the compound into the plasmid, these Orientations, I and II, depend significantly on the superhelicity of the plasmid, used either in a superhelical circular (sc) or linear (lin) form.
This resulted in a sufficient incorporation rate of 15N in all tissues in adulthood.
The experimental design required incubation time for sufficient incorporation.
and sufficient incorporation Cy3 (550 nm) and Cye5 (650 nm) dyes was verified.
To achieve a sufficient incorporation of DHA into tissue cell membrane phospholipids, an amount of 1.8 g/day of DHA was chosen on the basis of earlier studies carried out in healthy individuals and recently reviewed (Arterburn et al, 2006).
Although FISH-MAR can be applied successfully in anammox bacteria detection with insights of their function, a longer incubation time is necessary for sufficient incorporation of the labeled substrate, which might not always reflect the physiological state of anammox bacteria at in situ condition and/or at the time of sampling (Schmid et al. 2005).
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Using EGFP as a model protein, we have optimized this encapsulation system and found that fusion to the C-terminus of VP2/3 is preferable and that the C-terminal VP1-interaction domain of VP2/3 is sufficient for incorporation into VLPs.
Two different aaUTP salts were compared and both enabled sufficient fluorophore incorporation providing easily detectable microarray signals.
Cultures were grown for a 12 h period in the light to allow for sufficient isotope incorporation before first sampling at 1 h after the onset of the dark period.
Only 15 min of feeding on EdU/BrdU-containing bacteria was sufficient for measureable incorporation (Crittenden et al. 2006; Michaelson et al. 2010).
Taken together, these data suggest a concerted mechanism for enhancing net cholesterol absorption and minimizing its elimination to ensure sufficient cholesterol for incorporation into milk and bile acid synthesis, both important factors in maintaining the health of both the dam and pups.
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