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These two-tier delivery systems enable synergy between the delivery systems and may prove necessary to achieve sufficient gene expression to provoke robust cellular and humoral immune responses [58, 61 63].
The high efficiency of expression knockdown was reproducible with more than 70% of the F0 individuals showed sufficient gene expression suppression (<30% of the controls' levels).
The shortest deletion construct, plucFTP-Δ4, did not induce sufficient gene expression (below 1%; Fig. 5B).
ENIGMA can be used for any organism for which there is sufficient gene expression data available.
The rats were allowed to recover for up to 3 days to enable sufficient gene expression.
In this case the presence of multiple CDS (with both high and low CAI values) assigned to this function probably allowed a sufficient gene expression level to observe the expected phenotype.
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Pairwise variation analysis showed that the V2/3 value was 0.0126, which indicated two reference genes was sufficient for gene expression normalization and the two stability reference genes selected were EF1 and TUBA.
In this study, pairwise variation analysis (Fig. 4) showed that the V2/3 value was 0.0126, which indicated that two reference genes was sufficient for gene expression normalization and the two stability reference genes selected were EF1 and TUBA.
The results of this study verified that the −989/−318 promoter region of PB103464.00.0 is sufficient for gene expression to be restricted to the liver stage.
This raises the question if the shorter reads obtained with the more affordable sequencing technologies are sufficient for gene expression studies – i.e.: could they be mapped to the corresponding genes with a similar efficiency as longer sequence reads?
Growth in the more impoverished soil medium was slower than observed for J2315 grown in CF medium, but at least a single doubling was observed which was sufficient for gene expression under the soil conditions.
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