Together, these results demonstrate that a peptide consisting of 80 amino acids of domain III of eEF1A is sufficient for EPG modification in T. brucei.
In the case of sumoylation, loading appears to be both necessary and sufficient for the modification (Parker et al. 2008), whereas ubiquitylation is induced by a recruitment of Rad18 to loaded PCNA via interaction with RPA-coated ssDNA (Davies et al. 2008; Niimi et al. 2008), and phosphorylation by EGFR also occurs preferentially on chromatin-associated PCNA (Wang et al. 2006).
The interfacial stress and the pressure on the surface layer have been found to reach a few gigapascals which is sufficient for copper surface modification.
Using our assay conditions, the 2hr reaction time appears to be sufficient for the maximal modification with both donor substrates.
If simultaneous firing of hippocampal neurons during explorative-learning behaviour is sufficient for temporary synaptic modification, then the question arises as to whether similar cooperative firing can trigger persistent alterations in synaptic strength.
We consider a 12-week period sufficient for the behavioral modification component to wear off.
However, classic SUMO consensus sequences are neither necessary nor sufficient for determining SUMO modification of a protein.
Other viral vector systems have had limited application for T cell therapies, but Adenovirus has been used for transient episomal gene expression sufficient for T cell modification, as recently reported in a study engineering T cells to resist HIV-1 (Tebas et al, 2014).
Therefore, a more likely possibility is that residual FACT from the initial trophozoite-like stage is sufficient for any possible chromatin modifications that are needed for transcription of gametocyte-specific genes.
Nevertheless, aerobic exercise is central for exercise programmes aimed at reducing visceral adipose tissue, and aerobic exercise below current recommendations for obesity management may be sufficient for beneficial visceral adipose tissue modification [ 73].
The reduced activity of Chk1D469G may be sufficient for lesions arising from base modifications or strand breaks, but not for conditions that generate collapsed forks or unligated DNA.
