To create a sufficient dark-field background, the weakly scattered pump photons that do not obey TIR are also rejected by an additional absorption filter (see Fig. 1), as a result of which only the fluorescent emission from the objects is detected by the opto-electronic sensor-array.
Moderate cooling is sufficient to reduce dark counts to negligible levels.
The majority of parents take their young children out at the beginning of sunset, to let the kids have a sufficient time before dark.
While it is true that my high school courses in psychology, government and economics probably did not give me a sufficient view of the dark side of humanity, reading "The Brothers Karamazov" and studying the Holocaust certainly did.
And even those without sufficient knowledge of the dark arts to appreciate effective scrummaging could enjoy the sight of Adam Jones twice popping up at the base of a ruck in the first half to play the scrum-half's role.
In this paper we will show that some distortions of the Hermann grid display, which are sufficient to cause the dark gray smudges to entirely disappear, do not decrease the detection threshold of the disk.
A solution of 2 N HCl with 4 mM Fe(II SO4 reacted overnight and in the dark was sufficient for full reduction of 2 mM Mn(IV).
Leaf disks (10 mm diameter) obtained from new fully expanded leaves were immersed in H3PO4 (pH 4.0), HCL (pH 4.0), H2C2O4 (40 mM, pH adjusted to 4.0 with KOH), 40 mM K2C2O4, or different concentrations (20, 40, 60 mM) of K2C2O4 solution for 3 hours in the dark for sufficient permeation, and then floated on the solution for photoinhibition or recovery treatment.
In this way, we prevented re-absorption problems in a dense culture and provided sufficient illumination in the dark-acclimated samples (with an oxidized plastoquinone pool).
These diurnal patterns are consistent with previous investigations (Nozoye et al. 2004, 2011), and may be summarised as a slight induction of expression prior to the onset of light, a peak of induction 3 5 h after the onset of light, and a return to expression levels similar to that of Fe-sufficient roots prior to dark.
Reading and writing fantasy and dystopia isn't sufficient to address the darkest possibilities of human nature.
