In contrast to venous puncture as used to obtain sufficient blood to evaluate efficacy, the Dried Blood Spot (DBS) sampling method requires only a very small quantity of blood that is obtained by means of a finger prick.
Among other features, a high blood oxygen affinity assures a sufficient blood saturation.
All samples were tested for antibodies, and additionally for the presence of viral RNA if available blood quantities were sufficient.
Due to difficulties in obtaining sufficient quantities of blood from infants, the logistics of carrying out complete blood counts in the field or even using accurate blood volumes (10 µL) to make blood smears, we preferred to use the WHO recommended method of assumed leukocyte counts in such areas, also used in some IPTi studies [11], [44].
Therefore, this method is useful to detect mutations in newborns, from whom it is difficult to obtain a sufficient quantity of blood.
SmartAmp2 allows the analysis of mutations in many subjects, even newborns, from whom it is difficult to obtain a sufficient quantity of blood for genetic analysis.
These include limited laboratory facilities, difficulty in obtaining a sufficient quantity of blood from a small infant and suppression of bacterial growth in cultures by antibiotics given in labour [ 8].
Total RNA was extracted using the PAXgene Blood RNA Isolation Kit (Preanalytix), and sufficient RNA quantity, integrity or purity was required for inclusion.
From immunostaining, parallel loss of circadian photoentrainment and other adaptive ocular photoresponses, it is clear that DT can successfully cross the blood-retina barrier in sufficient quantity to trigger ablation of DTR expressing cells.
4, 5 Emerging concerns about the availability of sufficient quantities of safe blood products and transfusion-related risks led to the increasing use of erythropoiesis-stimulating agents (ESAs) in place of RBCTs.
Sometimes the antibodies don't get there in sufficient quantity or in sufficient time, and you end up amputating.
