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Despite numerous advances in the field, e.g. development of suitable methods for pulmonary administration of polymeric nanoparticles, a sufficient association of the therapeutic agent with the carrier system as well as drug release in a controlled fashion remain considerable challenges.
If all of the C-terminal 'tails' were exposed on the surface of a lamin filament that was capable of dynamic 'rolling' and situated adjacent to the surface of the INM then this might permit sufficient association with the processing enzymes.
CM is ranking first among the diseases related to MLANA according to the MalaCards and DISEASES databases, but its role as a risk gene for CM in MelGene currently remains unclear due to lack of sufficient association data.
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In fact, this region of Sestd1 associates with Dvl2 more robustly than the full-length Sestd1 protein (Fig. 1B, lane 4 vs. lane 5), suggesting that the C-terminal region of Sestd1 including the spectrin repeats, which is necessary and sufficient for association with Dact1 and Vangl2, inhibits association with Dvl2.
Thus, whereas the first 81 amino acids are sufficient for association with pol III subunits, the B box is required for association with Brf1, indicating that Maf1 interacts with pol III and Brf1 via different domains.
This suggests that the presence of the computationally inferred core motif is sufficient for association with human PUM proteins in vitro.
To establish whether the FYVE module was sufficient for association to the endocytic compartments, we created a series of truncated mutants in the upstream and downstream domains of AdcA (GFPFYVE-Y or FYVE-YGFP, GFPFYVE).
Using mutants of EHD2, we found that the formation of the 60 75S complexes was not sufficient for association with caveolae.
Moreover, the Ela1 C-terminal region was not only required, but also sufficient for association with purified Def11 500.
Generally, higher marker density is beneficial, although, if the extent of LD throughput the genome is high, fewer markers may be sufficient for association studies [ 35].
These figures describe a general trend that is useful for determining whether the average marker density is sufficient for association mapping purposes.
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Justyna Jupowicz-Kozak
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