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Previous studies have shown that a significant proportion of the more unstable 31A.2 fractures suffer from variable degrees of collapse and femoral shaft medialisation and that a high proportion of these patients go on to have poor function [5, 6, 30].
Importantly, revertible insertional mutagenesis is a more robust screening methodology than RNAi based approaches, which although flexible inevitably suffer from variable penetrance and off-target effects.
However, they suffer from variable properties and insufficient documentation of quality.
However, selecting improved variants from gene libraries in living cells requires plasmid expression systems that suffer from variable copy number effects, or the use of complex marker-dependent chromosomal integration strategies.
Finally, we used the φ C31 site-specific recombination system (Bischof et al, 2007), which allowed us to target transgene integrations to defined genomic locations (landing sites), an essential prerequisite for drawing conclusions from comparing the effects of modified and wild-type histone transgenes that could suffer from variable position effects if transgenes were randomly integrated.
The disadvantage of using short 60-mer probes is the low hybridization signal, which in theory is a factor of about 2000 times lower than when using the much larger insert BAC clones, which in turn suffer from variable but high DNA repeat content that must be quenched by Cot1 (the dominant fraction of human repetitive DNA sequence -DNA in the hybridization mix.
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However, it suffers from variable throughput depending on the noise levels and channel conditions [16,17].
The process suffers from variable quality in finished welds due to inherent limitations in the field.
Within each temporal replicate, 8 individually potted biological replicates of each variety were examined for each treatment except for B. nigra and B. juncea varieties, which suffered from variable germination.
If such variables are correlated with our tax measure, the baseline regression could suffer from omitted variable bias.
Unless funding is made available here to pay expert philosophy practitioners or to provide classroom teachers with rigorous training, our kids are condemned to forgo the many rich rewards that philosophy promises – or to suffer from the variable level of professionalism that characterises many volunteer-run educational programs.
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