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A 500-mL suction flask charged with 2.5 g Na+-montmorillonite (Southern Clay Products) and 10 mL CHCl3 was equipped with a constant pressure dropping funnel containing chlorosulfonic acid (0.50 g, 9 mmol) and a gas inlet tube for conducting HCl gas into water as adsorbing solution.
The following glass set was used to determine the imidazole released from the coordination sphere of the mixed complexes: suction flask, water suction pump, washer, Schott funnel POR 40 (see Additional file 4: Figure S4).
A 500-mL suction flask charged with 2.5 g Na+-montmorillonite (Southern Clay Products Inc., Gonzales, TX, USA) and 10 mL CHCl3 was equipped with a constant pressure dropping funnel containing chlorosulfonic acid (0.50 g, 9 mmol) and a gas inlet tube for conducting HCl gas into water as adsorbing solution.
- Suction flask: 250-ml or 500-ml filter flask.
Turn off the vacuum and remove the suction flask.
Fit the tetrafluoroethylene polymer disc on the upper part of the stem of the chromatographic tube, then place the tube with the disc on the suction flask and apply the vaccum (approximately 135-mm Hg pressure).
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Fit the funnel tightly against a filter flask via a rubber or synthetic rubber adapter to allow vacuum suction.
To synthesize TSB, each HCS was moved from the flask to a 6-well plate without touching the flask nozzle, using a 10 mL pipettor and gentle continuous suction.
More suction!
"More suction," he said.
In, suction, out.
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