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We sought to determine whether oropharyngeal sucrose exposure stimulates ingestive behavior in the near-term ovine fetus.
Ten breeding pairs of C57BL/6 mice (Jackson Laboratories, Ben Harbor) were set up and each litter was weaned at 21 days and distributed evenly between the experimental early environmental conditions (unlimited sucrose exposure and no sucrose exposure).
Five weeks after the termination of sucrose exposure, adult animals were tested for their willingness to work to acquire sucrose rewards in the progressive ratio test.
When high-sugar/high-fat dietary options were made freely-available, however, the sucrose-exposed mice gained more weight than mice without early sucrose exposure.
The sucrose exposure group, upon weaning, had 20 mg sucrose pellets (Bio-Serv, Frenchtown, NJ) continuously and freely available within their homecage.
Our data indicate that a single factor in early, post-weaning development– sucrose exposure– has persistent effects on adult motivated behavior and weight gain.
Similar(38)
The glucose challenge data suggests that the two groups respond to acute food deprivation (21 hour fast) and acute glucose increases similarly when maintained on standard chow, consistent with the observation that sucrose-exposure does not effect weight gain when animals eat standard chow.
Acute or continuous sucrose (1%) exposure did not affect motility.
In the current study, M sexta brains were removed, antennal lobes separated from the rest of the brains, both tissues homogenized and subjected to Triton exposure, sucrose density flotation, PAGE separation and immunoblotting as previously described [63].
Thus, Wfs1/CKO mice displayed a significant increase in immobility in the FST and a strong decrease in sucrose preference after exposure to ARS stress.
Therefore, the effect was dependent only on the time since the previous exposure to sucrose, demonstrating that sucrose consumption initiates a biphasic reduction in palatability.
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