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The SSR markers developed in this study provide valuable tools for genetic studies in pistachio and related species, such as germplasm characterization, fingerprinting of individuals, population genetics, genetic mapping and marker assisted selection, as well as integrating genetic linkage maps.
Originally, a privileged user would add genetic or genomic features, stocks (such as germplasm) and their respective genotypes in the feature, stock and genotype tables.
The 'Natural Diversity' module provides a set of templates for display of genotypic, phenotypic or other large-scale assays involving multiple features or stocks (such as germplasm).
These markers will be readily useful for the B. ruziziensis breeding program, aiding in areas such as germplasm characterization, construction of linkage and QTL maps, gene flow and mating system evaluation, and marker assisted selection.
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Allohexaploidy might also affect any potential manipulations of the C. sativa genome, such as introgression of germplasm or induced mutations.
We reported the development of 19 genomic SSR markers from enriched genomic SSR libraries in P. sibirica, providing valuable tools for genetic studies in P. sibirica and related species, such as population genetics, germplasm identification, and marker-assisted selection.
To achieve meaningful results, these approaches must be linked with suitable phenotyping protocols at all stages, such as the screening of germplasm collections, mutant libraries, mapping populations, transgenic lines and breeding materials and the design of OMICS and quantitative trait loci (QTLs) experiments.
In addition to the traditional gene-linked markers (Wang et al. 1994; Fjellstrom et al. 2006; Hayashi et al. 2004; Thakur et al. 2014), gene-specific or diagnostic markers were recently reported for the identification of novel blast R genes or alleles in diverse germplasm, such as Pi54 (Ramkumar et al. 2011), Pikm (Costanzo and Jia, 2010) and Pike (Chen et al. 2015).
The United States Department of Agriculture USDAA) participates in livestock preservation mainly through technology-based approaches such as gene mapping and germplasm (genetic material) storage.
Small and highly structured collections of germplasm, such as the CAP Core, are cost-effectively phenotyped and genotyped with high-throughput markers.
This cultivar was extremely popular in the former USSR but it has been replaced by more advanced cultivars based on the same germplasm, such as Saratovskaya 60 (released in 1995 [ 46]).
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