Fresh cells of C. termitidis were maintained by successively transferring 10%% (v/v) of inoculum to ATCC 1191 medium containing 2 g l−1 of cellulose, whereas fresh cells of C. beijerinckii were maintained by successively transferring 10%% (v/v) of inoculum to ATCC 1191 medium containing 2 g l−1 of cellobiose.
Vector pBBR1MC-5 [58], wharborsrbors mutS or mutL genes, was successively transferred into the different hypermutator P. aeruginosa CF isolates by electroporation as described by Choi and Schweizer [59].
Jiang et al reported that successively transferring the TargeTron-inactivated adc mutant in antibiotic-free CGM (Clostridium growth medium) for about 100 generations, the 1.5-kb intron fragment inserted into the adc gene was found to be lost in about 10% of the colonies, indicating the instability of intron insertion [51].
Spinal cords were removed, postfixed at 4°C and successively transferred to sucrose solutions (up to 30%).
In a typical phosphorelay, phosphoryl groups are successively transferred between T, R1, P and R2 domains.
A common example of an expanded TCS is the phosphorelay, wherein phosphoryl groups are successively transferred between His-Asp-His-Asp amino acid residues.
For cryoprotection, crystals of native Av1 grown at pH 9.5 were successively transferred into the reservoir solution with increasing concentrations of 2-methyl-2,4-pentanediol (MPD) up to 20%.
Unialgal cultures of the strains under study were obtained by micropipetting and/or streaking across agar plates (Hoshaw and Rosowski 1973), inoculated in tubes with f/2 medium and successively transferred to and maintained in Walne medium (Andersen 2005).
The slides were successively transferred to 3%H2O22 for 10 min and then incubated overnight with either rabbit monoclonal α-Ki-67 (M7187, Dako), α-LC3 (Novus Biologicals) or α-Caspase-3, which recognises cleaved Caspase-3 (Cell Signaling), after processing by EnVision system (Dako) according to the manufacturer's instructions.
