The supernatant was collected concentrated, and treated with 95% ethanol successively; the mixture was stored in a refrigerator at 4°C for 12 h.
Perhaps most enticingly, some physical properties of these devices that depend on their previous states can be successively tailored by the external stimulus and be stored even after power-off, very analogous to the behaviours of the biological synapse.
This new value is stored and new divisions are tested by successively increasing the length of the first frame by 5 ms and reducing the second one by 5 ms.
Parasternal (long and short axis), apical four-chamber, and subxiphoid four-chamber views were obtained successively in 2D mode, and a cine-loop was stored for offline analysis.
We conclude that in case of deceased or lost-to-follow-up patients, DNA can be retrieved from successively dialyzed, concentrated, and freeze dried urine that has been stored for up to 28 years.
We compared reproductive traits, such as duration of precopulation time, mating duration, quantity of sperm stored by females after copulation, number of females mated successively and postmating competition of irradiated males with non-irradiated males.
The stored images of each patient were blindly scored by the same investigator (MG) who successively performed the corresponding US lung examination two weeks after the baseline assessment.
Subsequently, tissue was successively infiltrated with 10 and 20%% sucrose in PBS, frozen in liquid nitrogen-cooled 2-methylbutane and stored at −80 °C.
After centrifugation, cells from the upper layer were successively collected, washed in PBS, centrifuged at 300 g for 10 min at room temperature, fixed in 4% paraformaldehyde/PBS solution for 15 min at room temperature, smeared on SuperFrost®Plus glass slides (Menzel Glaser, Braunschweig, Germany), air dried and finally stored at -20°C until further analysis.
