In 1 ml extract solution (1 mg/ml), 4 ml distilled water and 0.3 ml sodium nitrate (50 g/L) were successively mixed.
A laser beam of 633 nm operated at 17 mW was focused on the specimens and provided the excitation source. 2 µl aliquots of each 100 µl pellet suspension described earlier were successively mixed with 14 µl of 50 mM EDTA and 4 µl of concentrated "loading buffer" (0.313 M Tris-HCl pH 6.8, 10% SDS, 0.05% bromophenol blue, 50% glycerol, 12.5% β-mercaptoethanol).
Poly content was estimated by colorimetric assay based on oxidation-reduction reactions with Folin-Ciocalteu reagent [ 38, 39]. 100 μL of phenolic extract, 500 μL of Folin-Ciocalteu reagent (Sigma, diluted 10 times in ultra-pure water) and 400 μL of NaCO3 75 g.L-1 were successively mixed and incubated for 5 min at 40°C.
The average sum rates of the considered transmission schemes are shown in Fig. 4. Comparing the sum rates achieved in different interference scenarios, we can see that as the pico-BS moves, the system successively experiences mixed 2, strong, mixed 1, and weak interference scenarios.
Shoots (1 g FW) were homogenized in 5 ml cold acetone and then centrifuged at 10,000 × g for 10 min. To 1 ml of the supernatant aliquot, 3 ml of extract containing a 3 1 (v/v) ratio of CCl4 and CHCl3 and 5 ml of distilled water were added and mixed successively, and then centrifuged at 4,000 × g for 1 min. The supernatant was removed and tested for H2O2.
Table 4 gives the results of scale validity as a diagnostic method for NP, comparing its properties first in the total sample of NP patients, and then after successively removing patients with mixed pain and with central NP.
To study the recyclability of the catalyst, 1000 ppm mixed model oil was used successively seven times and the catalytic activity decreased a little (Fig. 4).
The alloys Mg-x at.%Ni (x = 33, 38, 43 and 50) with different nanometer-scale structures were successively synthesized by mechanical grinding of Mg2Ni mixed with various amounts of additional Ni, and the relations between their structural and hydriding properties were investigated in detail.
During hypothermia, SV decreased successively with increases in heart rate, and in the mixed model analysis, heart rate had a significantly greater negative effect on SV during hypo than during normothermia (Figure 2).
The second subroutine is used for sampling successively the vector of unknowns without setting-up the mixed model equations, thus accelerating considerably the performance by iteration.
An appropriate EspP derivative, SurA and proteoliposomes containing Bam complex were added successively to 20 mM Tris HCl pH 8. Reaction components were mixed after each addition.
