Sentence examples for successively inserted from inspiring English sources

To save the user's edge information and access the two vertices of the edge, they are successively inserted into the queue.

Both anti-sense and sense versions of a specific 780 bp fragment from the coding region of the TCD10 were amplified, and successively inserted into pTCK303, to form the RNAi construct vector pTCK303-dsRNAiTCD10.

Both anti-sense and sense versions of a specific 414-bp fragment from the coding region of the ASL3 were amplified, and successively inserted into pTCK303, to form the RNAi construct vector pTCK303-dsRNAiASL3.

To generate a BamHI site for mini-F sequence transfer into the terminal junction, 0.7 kb PCR-fragments amplified from both genomic ends using primers M and N or O and P (Table S1), were digested with SalI or SpeI and successively inserted into the XhoI or XbaI site of pCeu2.

The two fragments were successively inserted in-frame into pCRII and the reconstituted MITF-A cDNA with the 5′ UTR was subcloned into pcDNA3 vector (Invitrogen) at the XbaI and HindIII restriction sites.

Using the two sets of restriction endonuclease— Kpn I/ Xho I or Xba I/ Cla I, the 423 bp of partial CP gene and its reverse compliment fragment were successively inserted into each intron site contained in pHANNIBAL and enabled the hairpin intermediate vector pHANNIBAL-CP to make a hairpin loop).

We are successively, and successfully, inserted into the worlds of four male characters who seem to have little in common but who, in their anxiety and loneliness and essential decency, grow more and more similar.

Using the two sets of restriction enzymes, Cla I/ Xba I and Xho I/ Kpn I, successively, the target sequence was inserted into the two sides of the intronic region of pHANNIABL vector.

Then they were ligated successively by overlap-PCR and finally inserted into T-easy vector.

(PEO-CTA n was synthesized first by couPEO-CTA nα,ω-dihydroxyl PEO wash dicarboxylic trithiocarbonate, then synthesized) and t-butyl acrylate (tBA) were inserted into the (PEO-CTA)n successively to yield (PEO-b-PS)n and (PEO-b-PS-b-PtBA-b-PS)n, respectively.

Alternatively or additionally, the recombineered fusion with gfp inserted before the termination codon was secondarily modified with single nucleotide changes in starting exons to successively eliminate reporter expression arising from alternative transcripts.