All GLMs were perfomed in a stepwise manner with backward elimination; starting with the full set of predictive variables, we successively dropped those with a probability > 0.05.
Generally "touchdown" methods were used in which annealing temperatures were successively dropped in increments of 2-3°, from about 58 50° for Cytb and 62 55° for ND2, with increasing numbers of cycles at each lower temperature for a total of 35 40 cycles.
Therefore, proteins were solubilized in water to a concentration of 1 mg/mL, successively dropped in 20 μL droplets onto glass slides and dried in a vacuum (Vacuum Concentrator 5301; Eppendorf, Germany) at room temperature until achieving layers of required thickness [ 27].
These dominoes drop successively and lead, as if inevitably, to Christine's suicide.
The grids were subsequently washed by placing them, successively, on 10 drops of incubation buffer (5 minutes on each drop), after which the sections were incubated with the gold-conjugated secondary antibody by letting them float on drops containing the gold conjugate reagent (diluted 1∶20 in incubation buffer) for 60 minutes at room temperature.
In this case, the d f value drops successively among the different studies.
The droplet model is used to create a DFGM by successively solving the 1D flame equations at varying drop sizes, where the source terms for energy, mixture fraction (Z), and progress variable are cataloged as a function of Z and droplet diameter.
The normal braking system of freight trains is based on depression of a single brake pipe and its slow propagation towards the end of the train, successively putting all distributors into service when they detect the pressure drop.
Successive emanations of aeons were charged with successively diminished force.
A study from Norway of people aged 70 and over showed a rapid decline in response rate from the age group 70-74 (76.6%) successively declining to the age group 95+ where the response rate dropped to 27.1% [ 37].
