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Through twelve rounds of successive selections, a pool of ssDNA containing sequences with higher binding affinity to the target cells has been enriched.
Our data shows that the present MACS-based method leads, after three successive selections, to a population in which about 70% of the cells stably express the transgene.
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However, successive selection (5 passages) overcame the deficiency; selected nematodes no longer exhibited reductions in phenotypic traits.
The successive selection schemes in [14] can decrease the search size.
It was passed through three successive selection cycles of two weeks each.
Here, the variability of the timbers' properties is evaluated as the basis for the successive selection process.
The quality indicators should reflect related changes in users satisfaction, resource usage and system transparency, which is necessary for successive selection of promising evolution direction.
This paper proposes a Successive Selection Method (SSM), which is based on the linear Response Surface (RS) function and orthogonal design.
This situation is similar with the energy efficient mode switching and user scheduling in MU-MIMO systems [14], where the complexity reduction is obtained through successive selection schemes.
The enrichment of the selection pool through successive selection was monitored by flow cytometry.
The outcome of these processes may be complex, although in general, we expect that successive selection for male repressors of Wolbachia can lead to long-term elimination of infections.
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