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Of the 30 nuclear SNPs, 26 (87%) amplified successfully, of which 25 were polymorphic (Table 1).
Of the 24 maternally inherited SNPs, 23 (96%) amplified successfully, of which only nine were polymorphic (Table 1).
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A total of 78 indels were successfully validated of which 66 were observed to be in length multiples of 3, ranging from 3 to 33 bp, and hence not expected to cause protein translation frame shifts.
Eight recombinant proteins were successfully expressed, of which 6 were soluble and 2 were insoluble proteins.
A total of twenty-six participants were successfully interviewed, of which 15 were female and 11 male.
In a total of 14,198 WRA deaths during 2,595,605 person-years of observation, 12,939 had VA interviews successfully completed, of which 1,222 (9.4%) were pregnancy related.
Of 27 markers tested, 17 were successfully amplified, of which 12 loci were polymorphic, five were monomorphic, and 10 did not amplify after PCR optimization (Table 1).
Of the 64 primer pairs tested, 27 were successfully amplified, of which 17 showed polymorphisms and 10 were monomorphic (Table 1).
Following the default parameters in the CLC Genomics Workbench, around 92.3% of reads per library was successfully imported, of which approximately 83.4% was mapped.
A total of 1304 gSNP markers from the 1536 SNP array (84.9%) were successfully genotyped, of which 795 exhibited clear segregation within the YI pedigree.
Hence, a total of 20,278 primers were successfully designed, of which maximum was the ISBP (7,401; ∼36%) followed by RBIP (4,801; ∼24%).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com