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These results indicate that the Pten gene was successfully deleted from growing oocytes of PtenloxP/loxP; Zp3-Cre+ mice.
However, 36 clusters were successfully deleted from the prophage-free strain MB001 (ΔCGP123 and ΔISCg12).
We demonstrate in this study that MCAs can be successfully deleted from L. mexicana promastigotes, generating viable null mutants.
The result shows that the FabB/F and FabZ genes have been successfully deleted from blood stage P. yoelii parasites.
qPCR analysis showed that Trf1 had been successfully deleted from heart, intestine, lung, skin, blood, liver, kidney, bone marrow, brain, and stomach (Fig 6B).
* Restriction enzyme sites are underlined in prime sequences To verify ORFV113 and ORFV116 genes were successfully deleted from OV-IA82 via the transfer vector pSPV-EGFP and the EGFP gene was incorporated into the OV-IA82 genome by homologous recombination; Southern blot analysis was performed on genomic DNA isolated from OV-IA82, OV-IA82Δ113 and OV-IA82Δ116.
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Log back in; if the user is no longer visible on the options screen you have successfully deleted them from the system.
Messages you successfully delete for everyone will be replaced with "This message was deleted" in your recipients' chats.
In this study, we successfully deleted the ldhA gene from B. subtilis strain AFY to construct the recombinant strain AFYL.
For example, Wang et al. [ 6] successfully deleted the ldh gene from genomic DNA in B. licheniformis by transforming protoplasts of the cells with a recombinant knock-out plasmid; however, the designed protoplast system was very complicated and the transformation efficiency was low [ 6].
DECKO successfully deleted genomic fragments ranging in size from 100 to 3000 bp in four human cell lines.
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