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Of those, 674 sequences were finally successfully annotated after enhancing the annotation by including the results of an InterproScan database search and the ANNEX augmentation procedure [ 26].
A total of 26,006 genes met specific annotation thresholds and were successfully annotated using Blast2GO.
14,536 singletons (25% of total singletons) were successfully annotated and 4,121 singletons (28% of annotated singletons) were assigned with E.C.
The multi-step annotation process resulted in 16% successfully annotated sequences with GO terms.
Altogether, 46,206 (26.27%) genes were successfully annotated based on public databases, and the low annotation rate may be attributable to the limited genomic information for tree peony.
Singletons made up ~74% of the reftigs in the full (annotated and unannotated) 80% transcriptome and less than half of the singletons were successfully annotated.
Approximately 78.5% of 5619 HT unique sequences were successfully annotated by Blast search using public databases.
The results revealed that among the 269 predicted genes, 212 were successfully annotated.
After scanning for InterPro domains, 280 of these genes were successfully annotated, and 215 of them could be assigned to Gene Ontology (GO) terms.
Gene-disease associations and protein protein interactions have been successfully annotated in a similar manner, which were deposited in Nanopub.org [47].
30,392 isotigs (73% of total isotigs) were successfully annotated.
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