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The probe technique originated from early attempts of Anton van Leeuwenhoek to contrast microorganisms under the microscope using plant juices, successful staining of tubercle bacilli with synthetic dyes by Paul Ehrlich and discovery of a stain for differentiation of gram-positive and gram-negative bacteria by Hans Christian Gram.
However, there has been no previous report of the successful staining of human PMNs with MitoAP-1.
The mistletoe lectin ML-I[ 7] yielded successful staining with all pre-treatments.
We feel that resected specimens provide more histologic material for successful staining than biopsies.
Nevertheless, following concentration by centrifugation, enough biomass was obtained for successful staining experiments.
Successful staining for all three antibodies was examined on 120 patients.
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The final image shows successful Gad1 staining in pink.
This successful Gad1 staining took nearly a year of work between troubleshooting the IHC, switching to FISH, and tweaking the FISH protocol.
In addition to two-photon confocal microscopy, NL1 also displayed successful mitochondrial staining under stimulated emission depletion (STED) nanoscopy in living cells.
Successful FANCD2 staining was performed in 96 of the 129 cases of the VUmc sporadic array block.
There are no published reports of successful annexin V staining of zebrafish embryos and unfortunately we were also unable to obtain reliable annexin V staining.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com