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This is illustrated by what was commonly regarded as one of the most theatrically successful sequences in Daisey's show – one that also turns out to be untrue.
The show's most successful sequences, which come after the intermission, are the simplest in concept, managing to turn the minimal staging into an advantage.
If crows still extracted tools and probed into the food container when food was not present, it would be difficult to conclude that successful sequences of behaviour when food was present were the result of goal-directed planning.
The successful sequences and insertions were confirmed by DNA sequencing.
About 2000 transcripts were identified for each library from 8000 successful sequences.
The score recorded, ranging from 0 to 14, is the number of successful sequences.
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The resulting 1,110 BAC clones were end-sequenced, with successful sequence being acquired from 851 clones.
Therefore, we employed two-stage PCR to increase our chances of successful sequencing, thereby achieving a relatively high success rate of 862/1,038 reactions, or 83.0%.
Each locus was quantified for PCR amplification success, which is defined as the recovery and successful sequencing of each locus for each species.
Sequencing success for each primer was calculated by dividing the number of successful sequencing reactions by the total number of sequencing reactions performed for that particular primer.
Sequencing success for each partition was calculated by dividing the sum of successful sequencing reactions of the forward and reverse primer by the sum of the total number of sequencing reactions performed for each primer.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com