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Lentiviral vector systems have been considered to be the most successful method for generating transgenic chickens through delivering genes to embryos in newly laid eggs (Byun et al. 2013; Kwon et al. 2004; Lillico et al. 2007).
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However, due to the lack of pluripotent embryonic stem cells for the rat, ENU-driven target-selected mutagenesis, also known as TILLING [ 4] has been one of the most successful methods for generating knockouts in the rat [ 5, 6].
Here, we report the successful adaptation of this method for generating complex tissues from diverse tissue fragments derived from various organs, including pancreatic islets.
Lempert, R. J. et al. A general, analytic method for generating robust strategies and narrative scenarios.
In addition, a synthetic method for generating 25-OCH3-PPD, if successful, would ensure the consistency, potency, and purity of the compound for human studies.
We observed that this method for generating NSC was not always successful, due to the inconsistent appearance of contaminating cells that outgrew NSC in many cultures (NSC contaminants) (Fig. S1H J).
The most successful method for getting the word out, however, has been radio.
The most successful method for extending an animal's lifespan is caloric restriction.
In utero gene transfer is a successful method for transferring genes to the developing fetus.
Then, the obtained results showed a successful method for amino-functionalization of the MWCNTs.
Stabilization by phosphorus could be a successful method for providing additional stability during thermal regeneration.
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