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The absorbance spectra measured from 230 to 400 nm showed a small peak in the UCNPs-Ab conjugate that matches well with the characteristic peak of pure antibody, which proved the successful labeling of antibody with UCNPs (Fig. S4b).
After successful labeling and confirming the chemical stability of the tracer at least for 24 h in PBS by TLC, we performed solid-phase in vitro binding assays (Figure 2) that demonstrated that IG3 retained the ability to bind to certain ECM components [10].
We aim to highlight the versatility of the pyrazolyl-containing chelators, which allowed the successful labeling of a wide range of relevant biomolecules, spanning from small lipophilic organic molecules, such as quinazoline derivatives or DNA-binders, to tumor-seeking peptides for in vivo receptor targeting.
Recently, the first successful labeling of rodents (Stable Isotope Labeling in Mammals, SILAM) and protein expression measurements were reported [7] [10].
Consistent with our previous experience in immunolabeling apical complex proteins, where antigen accessibility was often an issue [7], harsh extraction conditions were needed for the successful labeling of the intra-conoid MTs with the anti-TgICMAP1 antibody.
Successful labeling of monocytes was confirmed by performing cytospin analysis and observing fluorescing monocytes under a microscope equipped with a 550-nm filter.
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