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Gastroscopy and esophageal radiography were performed immediately after removal of the stent to rule out possible complications, such as bleeding, perforation, etc. Technical success was defined as successful insertion of the stent into the esophageal achalasia without any major complications, such as esophageal rupture or massive bleeding.
Successful insertion of eGFP was detected by PCR using primers embedded in the inserted sequence and genomic sequences (Fig. 3A,C; supplementary material Fig. S6).
Time from completion of draping to successful insertion of needle.
The parameters studied included Time from completion of draping to successful insertion of needle.
To ensure successful insertion of the needle into the vein, development of a robot with high positioning accuracy was necessary.
The genome of the mutant was sequenced to ensure the successful insertion of the DNA fragments into the Mtb genome.
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After the successful insertion on December 07 , 2015 and the start of regular data acquisition in Apr.
Plasmids were isolated using the Wizard plasmid purification kit (Promega), and Sanger sequencing confirmed successful insertions of sequences to vectors.
Secondary outcomes were evaluated as follows: time needed for successful insertion, number of insertion attempts, oropharyngeal seal pressure (OSP), ease of insertion, fibre optic check of the vocal cords through the devices, and presence of perioperative oropharyngeal trauma or gastric content aspiration/regurgitation.
Fibreoptic (FO) evaluation of the SGA's position was performed after successful insertion and determination of the airway pressures and tidal volumes.
After successful insertion, the cuff of the LMA-S was inflated to a pressure of 60 cmH2O.
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