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Exact(22)
Importantly, a fraction of the circulating particles contained vector-derived DNA, indicating successful complementation in vivo.
Successful complementation was assessed by genotyping using F1, R1 and T5 primers (Additional file 1: Table S1).
Successful complementation of the remaining quorum mutants (Table 3) are consistent with the idea that growth rate changes were consequent to quorum sensing.
Successful complementation and functional recombination was detected in triple-transgenic mice (using the reporter line ROSA26-LoxP-Stop-LoxP-EYFP [17]; ROSY) when (1) both NCre and CCre were targeted to oligodendrocytes by the PLP promoter (Fig. 1C E; Fig. S3) or when (2) both fragments were expressed in astrocytes driven by the GFAP promoter (Fig. 1F H; Fig. S5).
Whereas successful complementation of levQ or levS deletions was achieved by introducing a wild-type levQ or levS sequence on plasmid pMSP3535, efforts to clone the levT gene in E. coli in a configuration that would allow for expression were unsuccessful.
Successful complementation was confirmed by PCR.
Similar(38)
Of these cells, the number of cases in which GFP fluorescence was also observed in cells adjacent to the primary foci (X) was used to calculate the proportion of successful infection complementation events (X/n).
Successful protein complementation requires that nonfunctional fragments of TEVp have a low affinity, and therefore the probability of fragments associating and reconstituting a functional enzyme at low concentrations is minimal.
Most of these sperm cells also expressed RFP signals and were detected in the vicinity of the border between the egg and central cells, indicating that GAA is less functional than the other mAtGCS1 constructs which are successful in complementation, such as GHH, GHP and GPP (Figures S1, 4E and 4F).
Hundreds of studies testing individual genes have revealed >200 successful human yeast complementation pairs (Dunham and Fowler 2013).
Also, we confirmed that a successful interallelic complementation occurs between the extracellular domain mutant allele and the cytoplasmic kinase-inactive mutant allele of BRI1 in vivo.
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