Exact(3)
Reviewer's report 3 Frank Eisenhaber, Bioinformatics Institute, Singapore The authors propose another variant of the successful BLAST suite of programs for similarity searches among protein sequences.
The de novo assembly contigs were divided into two sets: 'with BLAST', which are the contigs that resulted in a successful BLAST hit and 'without BLAST', which are the contigs without homology in the protein databases.
To assign gene ontology terms to each annotated sequence, successful blast hits were mapped and annotated using Blast2GO for the entire assembled transcriptome with the annotation cut-off threshold set to 55 and the GO level weighting set to 5. The single lane of Illumina HiSeq2000 produced close to 128 million paired-end reads (2 × 101 bp).
Similar(57)
Hexanitromannitol (nitromannite) functions in the same manner and is used that way in a very successful blasting cap.
Successful BLAST-results were used to position the T-DNA on the physical map of P. trichocarpa.
Our work provides a possible way forward for detection of and reasoning about regulatory DNA homology, analogous to but going beyond the highly successful BLAST-based paradigm for coding sequences.
Two major types of failed primer pairs that comprise most of the failures are primer pairs that failed on agarose gels but were successful by BLAST and primer pairs that failed on BLAST but were successful on agarose gels.
In conclusion, the sensitivity of the HMM searches makes this method more successful than BLAST to identify ISs on metagenomic samples, generating less false positives and providing a better picture of the IS diversity.
Preheated air was first used by James Beaumont Neilson in 1828 in Glasgow, but not until 1860 did the Englishman Edward Alfred Cowper invent the first successful hot-blast stove.
The successful sequences were BLASTed against EST-SSR sequences for which primers had already been designed [ 24, 26].
The successful reads were BLAST-ed against the DEOP gene database (Bit score 50, minimum percentage identity 60%) and mapped to DEOP pathways.
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