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Finally, the buffering of highly-basic bentonite at neutral pH improved the success of isolation of microbial populations, but not DNA, from the bentonite matrix.
Enzyme digestion and LDSCs enrichment are the two crucial steps which determine the success of isolation and culturing of the neonatal liver-derived stem cell in vitro.
It has also been reported that the success of isolation of T. gondii cysts as well as the number of tissue cysts produced vary considerably with the intermediate hosts, being higher in sheep, goats and pigs [ 49, 57].
In this study, we used five mice per sample to inoculate pooled brain and heart tissue homogenate of seropositive chickens which might have increased our success of isolation [ 9, 10].
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Following incubation, IgG- B cells were analyzed by flow cytometry to verify the success of the isolation procedure.
Modeling ESBL-E colonization in a French pediatric unit described the success of contact isolation in preventing nosocomial transmission, but its overall ineffectiveness due to the high incidence of sporadic cases from the community [ 17].
To assess the success of the isolation procedure and identify which density gradient fractions were enriched in lipid rafts from YH16.33, a T-T hybrid cell line, following detergent-free isolation, we carried out raft-ELISA using monoclonal antibodies directed to Thy-1 and Ly-6A.2, two GPI-anchored proteins known to localize in lipid rafts [ 14, 15, 23].
For branch pieces, the success of re-isolation of L. serpens dropped as the sample incubation time increased, while S. sapinea was always successfully (100%) re-isolated (even 12 months after the inoculation).
They also support the view that estimating cross-transmission extent is key to predicting the relative success of contact-isolation measures.
In a nutshell: the European Union is of the firm conviction that a policy of constructive dialogue has more chance of success than one of isolation and embargoes.
The ultimate success of any islet isolation and transplantation is, of course, in the benefit to the recipient.
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