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Our study indicates very high success of assignment to cluster, but also that inclusion of outlier loci significantly improved support for assignments.
Similarly, Ford and Williamson (2010) found a lower success of assignment to hatchery-born, relative to wild, Chinook salmon and showed that this lower performance was due to a smaller effective population size in the grandparental generation (i.e., the breeders in hatchery) for the former, as well as to the higher level of relatedness among hatchery-born spawners.
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To be a successful interim manager you'll need to learn to block out the noise and focus on the outcomes that you've committed to achieving in order to guarantee the success of your assignment.
In general, success of the assignment approach was poor, with only 128 (34.6%) of the 370 individuals successfully assigned to the correct subspecies and only 31 of the 144 C. a.
The highest success of species-level assignment using BLAST with individual barcodes was obtained with matK (100%), followed by trnH-psbA (98%), and then rbcLa (93.7%; Table 2).
Assignment success with geneclass2 and structure was generally quite high when compared with parentage data (>90% success, Hauser et al. 2006), and simulations performed here also showed similarly high assignment success and low rates of assignment error.
Given the observed plateau of assignment success beyond 100 SNPs, the top 96 SNP markers were selected to form a marker panel for the subsequent production of a 96-plex genotyping assay.
Given the relatively low success in assignment of maternity, we could not confidently estimate the distribution of mating success of females.
Five-group differentiations gave assignment success of 91% for NW European populations, 72% for Middle East populations and 39% for Central-South Asian populations, indicating Middle East individuals are not reliably differentiated from either Europeans or Central-South Asians.
The metric we used for predicting the assignment success of barcoding markers also requires alignments.
Assuming a intraspecific barcode threshold of maximum 2% (K2P), the success of achieving congruent species assignments (Additional File 5) was 97.3% and 98% when mean intraspecific divergences values were compared (in case BR vs BM, Figure 1).
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