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As far as this stability is evaluated using Gibbs free energy value, ΔΔG (the subtraction value of ΔG related to the 5' ends of the antisense and sense strands) determines the proportion of RISC entry rates for the antisense and sense strands.
The simplest way is to use the absolute value of the subtraction value.
Values from the anterior commissure white matter tract were used for a background subtraction value.
Note that if the pixel value of the background image is brighter than that of the input image at a certain pixel, the subtraction value becomes a negative value.
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Those subtraction values are named simply the delta values that is obtained by subtracting the trapped signal with the reference signal pertaining to the situation when there is no trapping but only medium present at the focus.
IOZ was defined as the cortical area with the greatest subtraction values.
After background subtraction, values were normalized to the control (DMSO only) cells.
After background subtraction, values were quantile normalised by R software, bad spots were deleted and duplicate spots were averaged.
The minus value of subtraction PS indicates declined PS.
Antigen-specific IFN-γ responses were considered positive when values were >100 pg·mL−1 after subtraction of values obtained from unstimulated cells.
Triplicate means were normalized firstly by subtraction of values for cells without antibody, and secondly, by subtraction of values for mock transfected cells.
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