Average signal was background subtracted with local background subtraction (BeadStudio, Illumina), quantile normalised and logged (base2) in R.6 (http://www.r-project.org/).org/
Two cDNAs (LIM kinase and BUB3), which are differentially-expressed in the present malignant library, have also been found to be differentially expressed in a subtracted library representing cDNAs expressed in a pathologically homogeneous breast carcinoma specimen subtracted with cDNA from 50 000 ductal-carcinoma- in-situ cells microdissected from the surrounding normal tissue (Liu, 2001).
This subtractive hybridization was performed using sample cDNAs (tester) from inoculated plants, which was subtracted with cDNAs (driver) from non-inoculated plants.
The two background data sets are added and scaled to the source data when being subtracted with the Sherpa subtract command, or modeled with set_bkg_model.
The high frequency (HF) outputs of the two identical photodiodes (81% quantum efficiency, with home-made low-noise electronics) are added or subtracted with a passive circuit, amplified by a low-noise, HF amplifier and sent to a spectrum analyser giving us the spectral noise power (thereafter simply called 'noise'noise
The signal and background training samples used for the BDT are derived from the Λb0 → pK−π+π− control sample, since its kinematics and topology are similar to the decays under study; background in this sample is subtracted with the sPlot technique41, a statistical technique to disentangle signal and background contributions.
The Cerenkov is subtracted with the result that the scintillation signal is left behind.
Raw spectra were averaged and background subtracted with a spline function to obtain the χ(k) function.
After the original Nth frame is shifting, the predicted N + 1th frame is subtracted with original N + 1th frame of the video sequence.
