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Images were background subtracted using the BG subtraction from ROI plug-in for ImageJ.
The cDNAs were subtracted using BD PCR-Select cDNA Subtraction Kit (Clontech) in both forward and reverse directions.
Background was subtracted using a region which was relatively free from the cluster emission.
For microarray analysis, transcript levels were quantile normalized and background subtracted using the GenomeStudio Gene Expression Module v1.0 Software (Illumina).
The signal from our galaxy was subtracted using the multi-frequency data.
Linear leakage was subtracted using P/4 protocol.
The signal from the our Galaxy was subtracted using the multi-frequency data, seen below.
The recorded spectra were background corrected and a linear baseline was subtracted using MATLAB 7.8.0.
The blood contribution was subtracted using the plasma fractional volume obtained in the minimization process.
The background from each spectrum was subtracted using a Shirley-type background to remove most of the extrinsic loss structure.
The collected spectra were averaged and the background fluorescence was subtracted using an asymmetric least squares smoothing.
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