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MT1-MMP recruitment to invadopodia over time was measured as the background-subtracted fluorescence intensity of MT1-MMP-pHLuorin at each time point subtracted from the initial fluorescence intensity at the time of invadopodium formation.
These values were subtracted from the initial dataset (Supplementary Fig. S2) to give the final heatmap shown in Fig. 3 and from which positive protein-glycan interactions and signal intensities, as an indication of relative avidities, can be inferred.
This boils down to estimate a within differentiated model, i.e. a model where the mean of each variable has been subtracted from the initial values.
The amounts of excess solution MnTPyP+ and those eluted from the surface of the clay were grouped together and subtracted from the initial nominal amount originally used.
Once the noise covariance matrix is estimated with the proposed algorithm, this matrix is subtracted from the initial covariance matrix of the received signals, and then we use the MUSIC method to localize the sources.
The extrapolated fit of the deactivation process was subtracted from the initial rising phase of the tail currents.
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The background well values were subtracted from the 100% initial activity wells and from the sample wells.
To better focus on the viscoelastic force, we subtracted from the traces the initial (static) force, which, as shown in Fig. 3, is slightly different for each muscle.
Once this point was reached, the tubes were flushed according to either the conventional technique or the new, air-circulating technique (see below), following which the trial was terminated and the volume of fluid aspirated was subtracted from the fluid instilled (the initial 500 mL + the amount flushed) to obtain GRV.
The concentration and enzyme activity of refolded protein in the supernatant were determined and the amount of soluble protein was subtracted from the total proteins subjected to the initial refolding procedure to estimate the amount of aggregation.
First, we calculate the feature histogram using SLBP of the initial frames and subtracted from the frames when a hand enters in the face region.
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