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For the Raman spectra, the procedure is more complex, and the diamond background was subtracted by using the procedure of Dalou et al. (2015).
Initial ohmic leak currents up to 500 pS were subtracted by using the leak correction mode of the EPC9.
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It was approximated by the polynomial function and subtracted by using "baseline correction" functionality of the LabSpec software.
A biotin label was introduced into the sense strands of cDNA that must be subtracted by using 5′ biotinylated forward primer during PCR amplification to immobilize the sense strand onto the streptavidin-linked paramagnetic beads.
The fluorescent intensity was quantified on the unmodified original images and the background intensity was subtracted by using Image J. F59 staining was diminished significantly after 2 dpf, while S58 staining was reduced at a slower pace and lost after 5 dpf.
During preprocessing the residual water signal was subtracted by using a decomposition-fitting algorithm.
Uncompensated capacitive transients and linear leak were subtracted by using Cs+ concentrations between 5 and 20 m M. The current/voltage relation obtained in this way is quasi-linear at Vm more negative than EK.
The background of each image was subtracted by using Elements.
Offsets due to antenna replacements were subtracted by using an offset dataset provided by GSI [http://mekira.gsi.go.jp/JAPANESE/corrf3o.dat].
Nonspecific binding was estimated and subtracted by using lysozyme as a substitution of hAFP.
For each sample, results were documented as cycle threshold (CT) set to 100 relative fluorescence unit values of background subtracted qPCR fluorescence kinetics by using the MX Pro Stratagene analysis software, applying the adaptive baseline, amplification based threshold and moving average algorithm enhancement.
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