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Oscillation traces were collected from single mother machine traps using the background subtracted average fluorescence intensity of the entire trap.
Background subtracted average fluorescence intensity was computed using FIJI (Schindelin et al., 2012) and plotted as arbitrary fluorescence units as a function of time.
(C ) Background subtracted average signal in arbitrary unit (A.U). in the Cy5 fluorescent channel picked up by Drosophila (left) or human (right) TFIID is plotted against their respective BSA controls.
Following the technique described previously in Willowson et al., a low activity source with negligible attenuation and scatter was imaged, and the background subtracted average count rate per detector compared with the calibrated activity to derive the sensitivity factor in units of count s−1 MBq−1.
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Absolute fluorescence readings were background subtracted, averaged for the replicate set of samples from a total of four independent experiments for statistical comparison.
Following the procedure summarized in [ 26], the ASL images were corrected for spatial variation of coil sensitivity, pair-wise subtracted, averaged, and then converted to CBFASL maps.
It subtracts average wage growth excluding bonuses from the CPI inflation rate -- if the result is negative (below the blue line), then incomes are failing to keep pace with rising prices.
We then generated average normalized spot intensities following a common strategy by subtracting average hph intensity (220) and adding 2 standard deviations (60) of the average normalized hph intensity).
Calibrated IB is obtained by subtracting average pericardial IB intensity from average myocardial IB intensity of the anteroseptum or posterior wall.
The qPCR data are often normalized by subtracting average C t values from those of predetermined housekeeping genes, producing a Δ C t readout (Livak and Schmittgen, 2001).
Then a ΔΔCT value was calculated by subtracting average acetone data point from JH, SHE, LR or LS (see formula below for JH).
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