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This conformation would be difficult to achieve with a large purine base and could contribute to the subtle preference for pyrimidines at the first position.
Together, the data suggest a subtle preference for pyrimidines in the first position of the codon, and purines in positions two and three (Pedersen et al., 2003).
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The company would come to know the subtle preferences of each and every television viewer.
When the targets of a PCSK were explored individually many amino acid residues with only subtle preferences could be seen at other positions.
Because this assay is not quantitative, these results do not rule out caspase-2 possessing subtle preferences for particular residues in this position, such as its previously reported preference for valine [ 59, 60] The caspase-2 reporter strain was also transformed with a library encoding random residues in the P4, P3 and P2 positions.
Later screening of libraries for phosphopeptides that displayed optimal binding to 14-3-3s identified two consensus 14-3-3-binding 14-3-3-binding 14-3-3-binding 14-3-3-binding II (RX(F/Y)X(pS)XP), with subtle preferences and negative determotifss for the X residues (Yaffe, 2002; Yaffe et al., 1997; Obsilová et al., 2008).
Retroviral integration sites display a subtle sequence preference unique to each virus [42], [43].
An ecotype is defined as a genetically coherent population within a species, having a subtle niche preference, different from other conspecific populations, Until recently, the taxonomic structure of the genus Prochlorococcus was poorly defined though.
It appears more likely from later studies that segregation of sympatric species, other than the blackcap and garden warbler, is due to subtle habitat preferences rather than interspecies aggression.
Thus, small differences in the largely conserved binding pockets probably dictate the subtle binding preferences of these defence lectins.
Conservation of specificity extended to secondary modes of binding, and even subtle dinucleotide preferences, suggesting that TF binding specificities are not subject to substantial evolutionary drift.
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