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The phrase 'subtle difference between' is correct and usable in written English.
You can use it when you want to describe something that has small variations that set it apart from something else. For example, "There is a subtle difference between the two versions of the song."
Exact(59)
The subtle difference between GA and GB (which only differ by a single hydroxyl group at position 1; Fig. 1 A) allowed us to use mutant cycle analysis to probe for direct interactions with the 2′ residue (24).
There's a subtle difference between "please" and "impress".
'There is a subtle difference between the two.
They learn to decipher the subtle difference between pain and injury.
We focused on a subtle difference between the two "bad" decks.
There is a subtle difference between decriminalisation - which Amnesty is advocating - and legalisation.
A film that we hope surfs the wave of subtle difference between our two countries, until it crashes red and frothy on to both shores.
Many people, however, had a hard time recognizing the subtle difference between the male and female bats on the bathrooms' swinging doors.
Barbados is also a generator of soca hits; Carnival connoisseurs can spy the subtle difference between Bajan soca tunes and Trini ones (hint: Bajans keep catchy choruses curt).
In other words, there is a subtle difference between crises associated with financial-market hubris and those associated with central-bank incompetence.
First of all, I have failed to recognise the subtle difference between ordinary, run-of-the-mill, Neapolitan pizza bread and its evidently more refined and fabulous baker brother schiacciata, a Tuscan flat bread.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com