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The pelvic fins are modified to form a sucker for attachment to the substratum (Figure 6c).
By 48 h, multiple individual depressions had fused (Figure 1f) and by 56 h, the cellulose substrate was dominated by large, irregular (approximately 200 μm) depressions into the substratum (Figure 1g).
It is noteworthy to emphasize that biofilms from upper section of the mask often appeared as peeling black thin crusts (Figure 2A, upper arrow) while biofilms from the lower section were heavier, moister and also were firmly attached to the substratum (Figure 2A, lower arrow).
By 24 h, there were markedly fewer cells, and extensive debris was seen attached to the substratum (Figure 3B).
In contrast, NMMIIA-deficient cell did not round up, and remained spread over the substratum (Figure 10).
Similar(55)
Defibrinated rabbit blood positively influenced the replication of anthrax spores compared to the other substrata (Figure 2).
Bourget (1987) describes this region as unattached in all barnacles; in A. amphitrite this region of the membrane is more readily lifted from the substratum (eg Figure 5) than the portions of the membrane that are nearer the most recent set of capillary ducts.
However, unlike SCp2 cells, loss of LM1 signalling in EpH4 cells induced stiffening and spreading comparable with that found on glass substrata (Supplementary Figure 2).
The sample 1corresponding to biofilms covering hard substratum (lower arrow, Figure 2A) and the sample 2 to biofilms coats softened stucco (upper arrow, Figure 2A).
Similar to TNT structure, EP bridges hovered above the underlying substratum of ECs (Figure 1L).
Moreover, when HIEC/shCNS cells were seeded on a type-I collagen matrix, a non-RGD substratum, the number of vinculin-positive FAs was significantly reduced compared with paxillin-positive FAs (Table 1 and Supplementary Figure S2E), closely recreating the phenotype of α8-knockdown and Y-27632-treated wild-type HIEC cells.
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