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Wound healing proceeds via fibroblast migration along three dimensional fibrillar substrates with multiple angles between fibers.
However, if enzyme mixtures, such as human fiver microsomes, are to be used probe substrate choices are more limited, as substrates with multiple pathways of metabolism are usually inappropriate.
Particularly important given the growing interest in enzyme conjugated and bispecific antibody therapeutics was the successful ligation of whole antibodies without disruption of the associations between their heavy and light chains or constant domains, and the fusion of multiple triglycine reactants to substrates with multiple LPETG motifs (Fig. 3A).
The tolerance to single nucleotide substitutions suggested that the Tev-mTALEN could retain high activity on substrates with multiple substitutions in the DNA spacer.
In complex substrates with multiple alkenes, this preference ensures that the least substituted alkene is always the better ligand for the Pt II) initiator, and consequently the site at which all electrophilic chemistry is initiated.
SH2 domain-containing tyrosine kinases have been proposed to use their SH2 domains to bind primed substrates to facilitate multisite (processive) phosphorylation of substrates with multiple tyrosine phosphorylation sites (Mayer et al., 1995).
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Glabridin is a natural substrate with multiple biological activities.
Glabridin is an originally natural substrate with multiple biological activities which propose it as a novel anticancer, antimicrobial and antifungal agent.
This suggests that during this event growth occurs through regime II kinetics whereby large numbers of surface nuclei form on the substrate, with multiple nucleations acts commencing before the previous ones have finished.
Finally, the separated liposome was decomposed, liberating the encapsulated glucoamylase to catalyze the hydrolysis of the signal substrate amylose with multiple turnovers, producing a large amount of glucose for quantitative readout by the PGM.
Contrary to Atg19, a substrate adaptor with multiple LIR motifs, p62 contains only a single LIR-motif and a single UBA-domain.
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