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CATL as well as NE were initially described in cytoplasmic vesicles as proteinases that degrade protein substrates with broad specificity and that both have well-defined elastinolytic and collagenolytic activity 109, 124– 124.
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Our work may establish an industrial procedure to produce complex hierarchical architecture on the high curvature surface of the flexible substrates with broader range of practical application.
To explore whether MMP activity was also increased in the ML-II embryos, total MMP activity was assayed using a FRET-based substrate with broad specificity for this class of proteases.
Furthermore, it was illustrated that iridium oxide substrates with relatively broad distributions of surface energies are suitable for both insect and vertebrate neuronal growth.
Given the diversity of lignin's monomeric units and the racemic nature of lignin polymers, we propose that bacterial catabolic pathways have overcome the existence of diverse lignin-derived substrates in nature by evolving multiple enzymes with broad substrate specificities.
HumanCyc uses classes as substrates in some reactions (e.g., an amino acid, an alcohol) as a way of catering for enzymes with broad substrate specificity or enzymes for which the exact substrate specificity is unknown.
This implies that an ancient enzyme with broad substrate specificity has progressively been evolved to gain narrower substrate specificity during evolution.
In addition, BGLs are divided into three groups on the basis of their substrate specificity, such as aryl-β-glucosidase, aryl- and alkyl-β-glucosidase, and enzymes with broad substrate specificity (Takahashi et al. 2011).
Subsequent characterization revealed YDR051c/Det1 to be an acid phosphatase with broad substrate specificity, whereas YOR283w has a broad pH range and hydrolyzes hydrophilic phosphorylated substrates.
It is suggested that phytases with broad substrate specificity are better suited for animal nutrition purposes than phytases with narrow substrate specificity [39].
Together, these molecules efficiently generate plasmin, a serine protease with broad substrate specificity.
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