Sentence examples for substrates will be necessary from inspiring English sources
For a rational design-based development of chitinases for biotechnological applications, an integrated understanding of the different roles of chitinases and, thus, their natural substrates will be necessary.
To fully understand the mechanism of catalysis and substrate specificity, it will be necessary to co-crystallize the proteins (wild type and mutant drDDC) with an inhibitor or to perform molecular docking with consideration of protein conformational change.
Future identification of loss-of-function and gain-of-function mutants, as well as the identification of the natural substrate of PvNod41, will be necessary to understand better the functional role of this enzyme during nodulation.
A concerted understanding of various aspects of fungal chitinases, ranging from gene regulation and protein functions to biochemical features and substrate-binding properties of different chitinases, will be necessary for advancing chitinase research toward biotechnological applications.
Further characterization of substrate and sugar specificity of litchi UFGT will be necessary to investigate.
Because the current structure is not a substrate-bound complex and a fragment near the active center is missing, further studies will be necessary to fully understand the substrate selection/recognition mechanisms of drDDC.
Other approaches such as immunoprecipitation using the cleaved-Caspase-3 antibody will be necessary to identify this substrate.
Additional studies will be necessary to validate putative BACE1 substrates in vivo and thereby clarify the role of BACE1 in the presynaptic terminal.
The feasibility of the FRET immunosensor technique was established; however, it will be necessary to immobilize the complexes onto optical substrates so that consistent trends can be obtained that would allow calibration plots.
New and more powerful Lewis acids will be necessary, however, to significantly broaden the substrate scope of catalytic carbonyl olefin metathesis.
If the slope is greater than 15 degrees (about 2/12), then a latticework frame that creates 2 × 2-foot or smaller boxes will be necessary to ensure there is not slippage of the substrate material.
