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When the MPTS-modified substrates were soaked in a vertical manner, square-like microcrystals with the (100) orientation were synthesized; however, when the substrates were soaked in a horizontal manner, triangular-like microcrystals with the (111) orientation were obtained.
After the temperature kept stable at 90 °C, the seeded substrates were soaked vertically in the solution for a period of 40 min.
The bare gold substrates were soaked into a hot piranha solution (v/v H2SO4:H2O2 = 3 1) for 30 min to clean the surface.
For pasteurization of the substrates, all the six substrates were soaked overnight in water following the tissue softening at 100 °C for 1.5 h, and finally were drained (Jafarpour and Eghbalsaeed 2012).
The substrates were soaked for 1 h in a solution made of 4% (v/v) of trimethoxyphenylsilane in analar grade isopropanol, IPA.
Air plasma-cleaned Ti6Al4V substrates were soaked in 3 mM of PA O Br/methanol solution for 24 h and then annealed at 110 °C to immobilize PA O Br on the surface.
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Thirty grams oat substrates was soaked in 30 mL UPW, DOW, SW, or various salts water, and then was autoclaved for 20 min at 121 °C in a 500-mL glass bottle.
Afterwards, the substrate was soaked in a solution of hydrophobic (or hydrophilic) surfactant molecules which filled in the un-stamped area.
The optimal process was that the substrate was soaked with an HCl concentration of 0.6 wt% at 90 °C for 2 h, and subsequently enzymatic hydrolysis for 72 h at 50 °C and pH 4.8 before fermentation.
After exposure to UV light under a photolithographic mask, the substrate was soaked in developer solution.
To assess ALN release from ALN (0.1 mg)/BCP and ALN (1 mg)/BCP, each substrate was soaked in a 15 mL conical tube with 1 mL PBS.
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