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Reactions omitting substrates were run under the same conditions as controls.
Here also, controls omitting one of the substrates were run with each assay.
Controls omitting one of the substrates were run with each assay.
Preliminary trials were made to determine optimal concentrations of reagent, and suitable controls lacking substrates were run to correct for non-specific activity.
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All samples for SDS electrophoresis were equilibrated to a final ACE activity of 200 mU/ml (Hip-His-Leu as a substrate) and were run using gradient (4 15%) Tris-HCl pre-cast SDS PAGE gels Bio-Red Laboratoriess, Hercules, CA).
Substrate and enzyme blanks were run at the same time by incubating the substrates without enzymes and by incubating the enzymes without substrates, respectively.
Also, several protein-substrate gel electrophoresis assays were run using gelatin, beta-gal, and bovine serum albumin (BSA) in the gel matrix, to determine the protein specificity of the proteases.
For all growth tests, uninoculated controls as well as cultures lacking the substrate to be tested were run in parallel.
ALD procedures were run at substrate temperatures of 150°C, 200°C, 250°C, 300°C, and 350°C, respectively.
ALD procedures were run at substrate temperatures of 150, 200, 250, 300, and 350°C, respectively.
Cerium oxide (CeO2) thin films used liquid injection atomic layer deposition (ALD) for deposition and ALD procedures were run at substrate temperatures of 150°C, 200°C, 250°C, 300°C, and 350°C, respectively.
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