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Substrates were released in up to 85% yield upon illumination with a high-pressure mercury lamp for 1.5 h.
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The first two substrates are released, but this ubisemiquinone intermediate remains bound.
The mechanism by which substrates are released from cortex, and in particular the role of multisite phosphorylation, has been unclear, however.
In order to prevent any misunderstanding we state in the legend for Video 1 that the order in which substrates are released is speculative.
Our aim in the manuscript was to discuss requirements for substrate release, rather than the exact physiological order in which substrates are released, which would be highly dependent on substrate concentrations.
Substrates are released directly from the excited state of the photocage within a few microseconds in the former case, whereas decarboxylation of photochemically generated monocarbonate in the latter case is a much slower process.
After the supplementation of XYL with AXE, 1.1% and 2.6% of acetyl groups (of the original xylan in the substrate) were released from wheat straw and giant reed, respectively.
Only after the first substrate is released can substrate B bind and react with the modified enzyme, regenerating the unmodified E form.
Autoinhibition of the catalytic triad in the absence of substrate is released by the binding of the Met1-linked proximal Ub.
Precise kinetic measurements of heterocyclization reactions are complex due to the solubility issues alluded to above and distributive processing by the enzymes, such that the substrate is released from the enzyme after each residue is heterocyclized.
With the HAT route, a hydrogen atom is transferred from the substrate to the mediator, as opposed to the ET route where only the electron is transferred to the mediator and the H+ ion from the substrate is released into the medium [ 17, 18].
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CEO of Professional Science Editing for Scientists @ prosciediting.com