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Finally, the substrates were precipitated in diethyl ether for 30 min at 4 °C and centrifuged.
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Excess proteins in substrate were precipitated by acid solution (HCl, 2 N).
Each single substrate was precipitated in cold ether and centrifuged.
Antibodies were precipitated using 3, 3′ diaminobenzidine tetrachloride as a substrate and the slides were counterstained using routine haematoxylin treatment.
HA-tagged Abl proteins were precipitated from HEK293 cells and assayed for phosphorylation of an optimal Abl substrate peptide.
Samples were precipitated by methanol/chloroform.
Proteins were precipitated using dichloromethane and methanol.
Caseins were precipitated from cell homogenate supernatants.
The modified RNAs were precipitated with ethanol.
Insoluble materials were precipitated by centrifugation.
RNA fragments were precipitated with ethanol.
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