Sentence examples for substrates were incubated from inspiring English sources

To measure the surface concentration of Fn, coated substrates were incubated in a solution of Fn antibody (1∶1000) followed by incubation with a secondary HRP-conjugated antibody.

100 mg substrates were incubated in 5 mL of 0.05 M sodium acetate buffer (pH 4.8) for 4 h.

The substrates were incubated with PLE for 48 h at 25 °C in borate buffer and samples were taken at predetermined intervals during the experiment.

Whereas FOR in the diet of donor animals did not affect methane:VFA ratio when HF substrates were incubated, for HC substrates methane:VFA ratio was greater (P<0.05) with inoculum from sheep fed alfalfa hay diets.

The substrates were incubated in a calcium phosphate solution for 1, 3, and 6 days (CPI, CPII, and CPIII respectively) at 37 °C to induce the formation of carbonated apatite.

Fig. 6 Adsorption isotherm of cellulosic substrates was at 4°. 100 mg substrates were incubated in 5 mL of sodium acetate buffer (pH 4.8) with various cellulase concentrations (~10 80 ul) for 2 h to reach equilibrium.

T1-treated virion RNA (Figure 7C) or full-length virion RNA (Figure 7D) from both W1-VPg31 and S1-VPgR1 radiolabeled substrates was incubated with an unlinkase source to determine if cleavage efficiency differed between the two viruses.

The substrates are incubated for an hour to allow sufficient time for the bacteria to adhere to the substrates.

To detect the enzymatic activity of IN by real-time PCR, 10 pM of biotinylated HIV-1 LTR substrates was incubated with 4  μM of purified IN enzyme in a reaction buffer at 37°C for 16 h.

Peptide arrays with 1176 different kinase pseudo-substrates were incubated with active c-Raf and MAP3K8 incubation mix (end concentration of 5 µg/ml active MAP3K8 kinase and 2 µg/ml of active c-Raf, 8% glycerol, 0.5 mM ATP, 10 mM MgCl2, 0.05% v/v Brij-35, 25 µg/ml BSA (Bovine Serum Albumin)) and 30 µCi 33P-γ-ATP, at 37°C for 60 minutes in a humidified oven.

For trap-assays, the enzyme and the RNA substrate were incubated on ice for 4 min, in the absence of ATP.